Work

Purification of micronuclei from cultured cells by flow cytometry

Year: 2021

Type: article

Abstract: Micronuclei are aberrant nuclear compartments that form when chromosomes or chromosome fragments fail to incorporate into a primary nucleus during mitotic exit. Ruptures at the micronuclear envelope are associated with DNA damage and activation of immune sensing pathways. To gain insights into these processes, we have developed a method to purify ruptured micronuclei. This method paves the way toward understanding the consequences of micronuclear envelope rupture. For complete details on the use and execution of this protocol, please refer to Mohr et al. (2021).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2021.100378

Authors: Eléonore Toufektchan, John Maciejowski

Language: English

Topic: Nuclear Structure and Function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500

Funders: Memorial Sloan-Kettering Cancer Center, National Cancer Institute