Comparing Transient Oligonucleotide Hybridization Kinetics Using DNA-PAINT and Optoplasmonic Single-Molecule Sensing on Gold Nanorods
Year: 2021
Type: article
Abstract: We report a comparison of two photonic techniques for single-molecule sensing: fluorescence nanoscopy and optoplasmonic sensing. As the test system, oligonucleotides with and without fluorescent labels are transiently hybridized to complementary “docking” strands attached to gold nanorods. Comparing the measured single-molecule kinetics helps to examine the influence of the fluorescent labels as well as factors arising from different sensing geometries. Our results demonstrate that DNA dissociation is not significantly altered by the fluorescent labels and that DNA association is affected by geometric factors in the two techniques. These findings open the door to exploiting plasmonic sensing and fluorescence nanoscopy in a complementary fashion, which will aid in building more powerful sensors and uncovering the intricate effects that influence the behavior of single molecules.
Source: ACS Photonics
Institutions: University of Exeter, University of Potsdam
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: EPSRC Centre for Doctoral Training in Electromagnetic Metamaterials, Molecular Mechanics of Enzymes, Nanoscale thermodynamics: From Expeanriments and Applications to a practical Theory (noFEAT), Phyto-optofluidics - A quantitative super-resolution imaging approach for next generation plant physiology research, A new super-resolution proximity assay to probe RNA transcription condensates, G6704715602, Focus enhanced single molecule super-resolution microscopy - correlative confocal and nanoscale imaging in thick tissues, An Optical Single Molecule Scanner of Protein Motion
OpenAlex ID: W3138464421