A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
Year: 2019
Type: article
Abstract: In organ transplantation, infection and rejection are major causes of graft loss. They are linked by the net state of immunosuppression. To diagnose and treat these conditions earlier, and to improve long-term patient outcomes, refined strategies for the monitoring of patients after graft transplantation are needed. Here, we show that a fast and inexpensive assay based on CRISPR-Cas13 accurately detects BK polyomavirus DNA and cytomegalovirus DNA from patient-derived blood and urine samples, as well as CXCL9 messenger RNA (a marker of graft rejection) at elevated levels in urine samples from patients experiencing acute kidney transplant rejection. The assay, which we adapted for lateral-flow readout, enables-via simple visualization-the post-transplantation monitoring of common opportunistic viral infections and of graft rejection, and should facilitate point-of-care post-transplantation monitoring.
Institutions: Max Delbrück Center, Massachusetts Institute of Technology, Charité – Universitätsmedizin Berlin, Brigham and Women's Hospital, Harvard University, Wyss Institute for Biologically Inspired Engineering, Boston Children's Hospital, Albert Einstein College of Medicine, Montefiore Medical Center, Broad Institute
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W3138893390