Work

ASHLEYS: automated quality control for single-cell Strand-seq data

Year: 2021

Type: article

Abstract: SUMMARY: Single-cell DNA template strand sequencing (Strand-seq) enables chromosome length haplotype phasing, construction of phased assemblies, mapping sister-chromatid exchange events and structural variant discovery. The initial quality control of potentially thousands of single-cell libraries is still done manually by domain experts. ASHLEYS automates this tedious task, delivers near-expert performance and labels even large datasets in seconds. AVAILABILITY AND IMPLEMENTATION: github.com/friendsofstrandseq/ashleys-qc, MIT license. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Source: Bioinformatics

DOI: 10.1093/bioinformatics/btab221

Authors: Christina Gros, Ashley D Sanders, Jan O Korbel, Tobias Marschall, Peter Ebert

Institutions: Saarland University, European Molecular Biology Laboratory, Heinrich Heine University Düsseldorf

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,478

Funders: Deutsche Forschungsgemeinschaft, National Institutes of Health, National Human Genome Research Institute