Escherichia coli Colonization of Intestinal Epithelial Layers In Vitro in the Presence of Encapsulated Bifidobacterium breve for Its Protection against Gastrointestinal Fluids and Antibiotics
Year: 2021
Type: article
Abstract: Encapsulation of probiotic bacteria can enhance their functionality when used in combination with antibiotics for treating intestinal tract infections.The interaction strength of encapsulating shells, however, varies among the encapsulation methods and impacts encapsulation.Here, we compared the protection offered by encapsulating shells with different interaction strengths toward probiotic Bifidobacterium breve against simulated gastric fluid and tetracycline, including protamine-assisted SiO 2 nanoparticle yolk-shell packing (weak interaction across a void), alginate gelation (intermediate interaction due to hydrogen binding), and ZIF-8 mineralization (strong interaction due to coordinate covalent binding).The presence of encapsulating shells was demonstrated using X-ray-photoelectron spectroscopy, particulate microelectrophoresis, and dynamic light scattering.Strong interaction upon ZIF-8 encapsulation caused demonstrable cell wall damage to B. breve and slightly reduced bacterial viability, delaying the growth of encapsulated bacteria.Cell wall damage and reduced viability did not occur upon encapsulation with weakly interacting yolk-shells.Only alginate-hydrogel-based shells yielded protection against simulated gastric acid and tetracycline.Accordingly, only alginatehydrogel-encapsulated B. breve operated synergistically with tetracycline in killing tetracycline-resistant Escherichia coli adhering to intestinal epithelial layers and maintained surface coverage of transwell membranes by epithelial cell layers and their barrier integrity.This synergy between alginate-hydrogel-encapsulated B. breve and an antibiotic warrants further studies for treating antibioticresistant E. coli infections in the gastrointestinal tract.
Authors: Lu Yuan, Hao Wei, Xiao-Yu Yang, Wei Geng, Brandon W. Peterson, Henny C. van der Mei, Henk J. Busscher
Institutions: University Medical Center Groningen, University of Groningen, Harvard University, Wuhan University of Technology, State Key Laboratory of Advanced Technology For Materials Synthesis and Processing, Sun Yat-sen University, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,000
Awards: G1578183256, G3754227356
OpenAlex ID: W3142601306