Work

Using delayed decoupling to attenuate residual signals in editing filters

Year: 2021

Type: article

Abstract: Isotope filtering methods are instrumental in biomolecular nuclear magnetic resonance (NMR) studies as they isolate signals of chemical moieties of interest within complex molecular assemblies. However, isotope filters suppress undesired signals of isotopically enriched molecules through scalar couplings, and variations in scalar couplings lead to imperfect suppressions, as occurs for aliphatic and aromatic moieties in proteins. Here, we show that signals that have escaped traditional filters can be attenuated with mitigated sensitivity losses for the desired signals of unlabeled moieties. The method uses a shared evolution between the detection and preceding preparation period to establish non-observable antiphase coherences and eliminates them through composite pulse decoupling. We demonstrate the method by isolating signals of an unlabeled post-translational modification tethered to an isotopically enriched protein.

Source: Magnetic Resonance

DOI: 10.5194/mr-2-475-2021

Authors: Kenneth A. Marincin, Indrani Pal, Dominique P. Frueh

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $992

Funders: National Institutes of Health, National Institute of General Medical Sciences