Year: 2020
Type: dissertation
Abstract: Detachment of fragile cell types cultured on two-dimensional (2D) surfaces detrimentally affects cell viability. Accutase treatment of neurons growing in 2D damaged fragile neuronal connections, resulting in cell death and host tissue damage in vivo. Avoiding cell detachment altogether by developing an in vitro culture system may better maintain cell viability; therefore, we present the synthesis of a reversible crosslinked three-dimensional (3D) hydrogel which would provide stability for culture/differentiation when crosslinked and injectability for transplantation following crosslink degradation. A blend of hyaluronan (HA) and methylcellulose (MC), HAMC, was chemically crosslinked with an enzyme-degradable peptide sequence using oxime click-chemistry. Specifically, HA and MC were modified with ketone and aldehyde groups, respectively, and a TEV protease-degradable peptide crosslinker was synthesized and modified with oxyamine groups. The gel components were characterized, and gels were formed that demonstrated good stability, improved injectability upon crosslink degradation, and cytocompatibility with iPSC-derived neural progenitor cells.
Source: TSpace (University of Toronto)
Author: Kelti Aileen Smith
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W3145341244