An ionic liquid supported CeO2 nanoparticles–carbon nanotubes composite-enhanced electrochemical DNA-based sensor for the detection of Pb2+
Year: 2011
Type: article
Abstract: An electrochemical sensor incorporating a signal enhancement for the determination of lead (II) ions (Pb2+) was designed on the basis of the thrombin-binding aptamer (TBA) as a molecular recognition element and ionic liquid supported cerium oxide (CeO2) nanoparticles–carbon nanotubes composite modification. The composite comprises nanoparticles CeO2, multi-wall carbon nanotubes (MWNTs) and hydrophobic room temperature ionic liquid (RTIL) 1-ethyl-3-methylimidazolium tetrafluoroborate (EMIMBF4). The electrochemical sensors were fabricated by immersing the CeO2–MWNTs–EMIMBF4 modified glassy carbon electrode (GCE) into the solution of TBA probe. In the presence of Pb2+, the TBA probe could form stable G-quartet structure by the specific binding interactions between Pb2+ and TBA. The TBA-bound Pb2+ can be electrochemically reduced, which provides a readout signal for quantitative detection of Pb2+. The reduction peak current is linearly related to the concentration of Pb2+ from 1.0×10–8 M to 1.0×10–5 M with a detection limit of 5×10–9 M. This work demonstrates that the CeO2–MWNTs–EMIMBF4 nanocomposite modified GCE provides a promising platform for immobilizing the TBA probe and enhancing the sensitivity of the DNA-based sensors.
Authors: Yan Li, Xiao-Rong Liu, Xiao-Hui Ning, Can-Can Huang, Jian-Bin Zheng, Jun-Cai Zhang
Institutions: Northwest University, Xianyang Normal University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,950
Award: G3349533787
OpenAlex ID: W260520484