Complement Receptor-mediated Phagocytosis is more Pro-inflammatory than Fcγ Receptor-mediated Phagocytosis in Murine Macrophages
Year: 2020
Type: dissertation
Abstract: Macrophages are immune cells capable of recognizing and removing foreign organisms through phagocytosis. It is widely believed that Fc receptor (FcR)-mediated phagocytosis is pro-inflammatory while complement receptor (CR)-mediated phagocytosis is not. Our work involved examining cytokine and chemokine levels, post-phagocytosis of opsonized sheep red blood cells (sRBCs), as a means of determining the murine macrophage’s inflammatory response. Interestingly, our cytokine array and ELISA data showed that CR-mediated phagocytosis is more pro-inflammatory than FcR-mediated phagocytosis in bone marrow-derived murine macrophages. We further explored the use of a physiologically relevant alternative CR activator, GM-CSF. We also determined that unbound sRBC decay does not stimulate cytokine production. Additionally, inhibitors of NF-κB, calpain and phagocytosis were used to determine their roles in the pro-inflammatory response that we observed in macrophages. Our data reveals that CR-mediated phagocytosis is more pro-inflammatory than FcR-mediated phagocytosis for opsonized sRBCs and is potentially mediated by calpain activation of NF-κB.
Source: TSpace (University of Toronto)
Author: Xiao Rui Li
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3150184284