The Effects of Aging on Mesenchymal Stem Cell Extracellular Vesicle Treatment following Myocardial Ischemia/Reperfusion
Year: 2020
Type: dissertation
Abstract: Extracellular vesicles (EV) have recently emerged as a key factor secreted by stem cells which improve infarct healing post-myocardial infarction (MI). However, the cellular processes which regulate EV function are largely unknown. Here we investigate young and old mouse bone marrow mesenchymal stem cells (MSCs) to determine whether aging impacts the beneficial effects of secreted EVs. Cells and EVs were studied in-vitro to determine the effect of aging on cell function as well as EV secretion. Using a mouse model of myocardial ischemia/reperfusion (I/R) we show that young, but not old, MSC-EVs preserve left ventricular function at four weeks post-I/R by echocardiography. Mechanistically, we found that young MSC-EVs modulate the inflammatory response more effectively than old MSC-EVs in-vitro and in-vivo. Understanding how aging affects EV secretion and function will lead to a better understanding of the cellular processes which regulate the beneficial effects of EVs on cardiac repair.
Source: TSpace (University of Toronto)
Author: Fievel Lim
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3150805586