An Integrative Modelling Approach for Disordered Proteins using Single-molecule Fluorescence Spectroscopy
Year: 2019
Type: dissertation
Abstract: The primary sequences of Intrinsically Disordered Proteins (IDPs) encode for relatively flat, but not featureless, energy landscapes. Consequently, characterizing their conformational properties is non-trivial due to their heterogeneity and interconversion of conformations. I developed experimental protocols and data analysis tools to study IDPs using single-molecule Förster Resonance Energy Transfer (smFRET) with Multiparameter Fluorescence Detection (MFD). Using multiple parameters of fluorescence is necessary to make accurate and unambiguous distance measurements using the mean transfer efficiency
Source: TSpace (University of Toronto)
Author: Gregory-Neal W. Gomes
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3153118786