PKCδ Regulates Actomyosin Organization in Leading Cellular Protrusions during Drosophila Border Cell Migration
Year: 2020
Type: dissertation
Abstract: Actin-based membrane protrusions are essential for cell migration, including the collective migration of the border cell cluster (BCC) during Drosophila oogenesis. Our lab previously showed that the serine/threonine kinase Protein Kinase C δ (PKCδ) regulates the structure and behavior of the leading protrusion of the BCC. Here, I investigated the influence of PKCδ on the actomyosin network of BCC protrusions. Examination of PKCδ loss-of-function mutants revealed that PKCδ is required for normal F-actin organization and Myosin II (MyoII) distribution in protrusions. Overexpression of PKCδ caused a severe reduction in actomyosin in protrusions. Moreover, PKCδ prevents simultaneous formation of multiple leading protrusions. Rho1, a positive upstream regulator of MyoII, appears to be activated in the leading protrusion precociously when PKCδ function is lost. In summary, my analysis contributes to our understanding of the function of PKCδ as a regulator of the actomyosin network in leading cellular protrusions during collective cell migration.
Source: TSpace (University of Toronto)
Author: Amad Imran Bhatti
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3154302758