Assessment of Bioaerosol Samplers for the Detection and Quantification of Influenza Virus and Compounds that Potentially Influence Influenza Virus Replication
Year: 2018
Type: dissertation
Abstract: Bioaerosol samplers are used to detect and characterize influenza virus bioaerosols but current guidelines for sampler selection are lacking. We examined four bioaerosol samplers using artificial bioaerosols and the ferret model of IAV infection. Samplers included a PTFE filter, NIOSH cyclone sampler, Andersen impactor, and SpinCon®. The PTFE filter and NIOSH cyclone sampler retained vRNA and infectious artificial bioaerosols. All bioaerosol samplers collected vRNA and the PTFE filter and NIOSH cyclone sampler retained infectious virus from infected ferrets. Ease of use, and infectious virus retention make the PTFE filter and NIOSH cyclone sampler suitable for the collection of IAV. We also examined the ability of digoxin to inhibit H1N1 replication. H1N1 viral loads decreased after incubation with digoxin while minimal cellular metabolic inhibition was observed. Digoxin in combination with oseltamivir, examined to determine the additive effects, also decreased viral loads in vitro. Digoxin appears to inhibit H1N1 replication in vitro.
Source: TSpace (University of Toronto)
Author: Christian Bekking
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3155816906