Year: 2014
Type: dissertation
Abstract: Smaug is a RNA-binding protein that regulates translational repression and/or degradation of its targets, and is required for the elimination of a large number of maternal transcripts in early Drosophila embryos. Here, I explored a novel role of Smaug in regulation of Smaug in early embryos. Previous experiments identified mRNAs encoding glycolytic enzymes as associated with Smaug. I found that mRNAs encoding these enzymes have predicted Smaug recognition elements (SREs) and are destabilized by Smaug. Furthermore, I performed enzyme activity assays and showed increased activity of several glycolytic enzymes in smaug mutants. Experiments comparing the metabolome of wild-type and smaug mutant embryos implicated Smaug in an array of metabolic pathways including glycolysis.
Source: TSpace (University of Toronto)
Author: Zhiyong Yang
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3155929398