Integrating Biomaterials and Genome Editing Approaches to Advance Biomedical Science
Year: 2021
Type: review
Abstract: The recent discovery and subsequent development of the CRISPR-Cas9 (clustered regularly interspaced short palindromic repeat-CRISPR-associated protein 9) platform as a precise genome editing tool have transformed biomedicine. As these CRISPR-based tools have matured, multiple stages of the gene editing process and the bioengineering of human cells and tissues have advanced. Here, we highlight recent intersections in the development of biomaterials and genome editing technologies. These intersections include the delivery of macromolecules, where biomaterial platforms have been harnessed to enable nonviral delivery of genome engineering tools to cells and tissues in vivo. Further, engineering native-like biomaterial platforms for cell culture facilitates complex modeling of human development and disease when combined with genome engineering tools. Deeper integration of biomaterial platforms in these fields could play a significant role in enabling new breakthroughs in the application of gene editing for the treatment of human disease.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Funders: National Science Foundation, Retina Research Foundation, National Institutes of Health, Advanced Research Projects Agency, National Heart, Lung, and Blood Institute, National Human Genome Research Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Biomedical Imaging and Bioengineering, Division of Materials Research, Division of Chemical, Bioengineering, Environmental, and Transport Systems, National Center for Advancing Translational Sciences
Awards: G1152963880, Systemic Inflammation in Microphysiological Models of Muscle and Vascular Disease, G1612249789, Epigenome Editing Technologies for Treating Diverse Disease, Enhancing Neuronal Reprogramming with Epigenome Editing, Collaborative Research: Adapting Cas9 Protein from CRISPR as a Structural Unit for Molecular Assembly, Combinatorial biomimetic systems to enable high-throughput evaluation and expansion of genome-edited dystrophic muscle stem cells, CAREER: Towards Synthetic Biology in Human Stem Cells, Regulatory Mechanisms of CD4+ T Cell Differentiation, Evolving High Potency AAV Vectors for Neuromuscular Genome Editing, Microphysiological Human Tissue Systems for Monitoring of Genome Editing Outcomes, EFRI CEE : Engineering Technologies to Determine Causal Relationships Between Chromatin Structure and Gene Regulation, CRISPR/Cas9-Based Gene Editing for the Correction of Duchenne Muscular Dystrophy, G792802007, The Duke FUNCTION Center: Pioneering the comprehensive identification of combinatorial noncoding causes of disease, Microphysiological Human Tissue Systems for Monitoring of Genome Editing Outcomes, Assembly of Novel Gene Editing Particles to Understand Genome Surgery in Patient-Derived Cells
OpenAlex ID: W3157469971