Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
Year: 2021
Type: article
Abstract: Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).
Source: STAR Protocols
Authors: Liliana Sokol, Vincent Geldhof, Melissa García-Caballero, Nadine V. Conchinha, Sébastien J. Dumas, Elda Meta, Laure-Anne Teuwen, Koen Veys, Rongyuan Chen, Lucas Treps, Mila Borri, Pauline de Zeeuw, Kim D. Falkenberg, Charlotte Dubois, Magdalena Parys, Laura P.M.H. de Rooij, Jermaine Goveia, Katerina Rohlenova, Luc Schoonjans, Mieke Dewerchin, Guy Eelen, Xuri Li, Joanna Kalucka, Peter Carmeliet
Institutions: VIB-KU Leuven Center for Cancer Biology, KU Leuven, University of Antwerp, GZA Ziekenhuizen Campus Sint-Augustinus, GZA Ziekenhuizen, Sun Yat-sen University, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Aarhus University, Université de Lille
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Funders: Aarhus Universitet, Aarhus Universitets Forskningsfond, Carlsbergfondet, Fonds Wetenschappelijk Onderzoek, Lundbeckfonden, Universiteit Antwerpen, Novo Nordisk Fonden, Kom op tegen Kanker, Vlaamse regering, Fondation contre le Cancer, Stand Up To Cancer, Aarhus Institute of Advanced Studies, Aarhus Universitet, H2020 European Research Council, Steno Diabetes Center Aarhus
OpenAlex ID: W3159348931