Protocol for characterization of morphology, electrophysiology, and synaptology of mouse stellate ganglion neurons v2
Year: 2020
Type: preprint
Abstract: The stellate is a thoracic sympathetic ganglion supplying autonomic innervation to cells, tissues and organs of the upper thorax; including, but not limited to, the brachial sweat glands, mammary glands, cardiomyocytes, and sinoatrial and atrioventricular nodes. We sampled individual cells of the mouse right stellate ganglion with intracellular microelectrodes to create a topographic atlas of ganglion neuron structure and function. Membrane potential recordings, during stimulation of presynaptic nerves, were paired with confocal imaging of single cells, backfilled with fluorescent dyes, to quantify cell morphology, membrane physiology, and synaptology of the individual cells. The cellular database, including localization and projection of each cell, will be shared publicly through a link in the manuscript, which will be appended to this protocol once published. In the accompanying manuscript, we describe unique populations of cardiac-projecting stellate neurons and detail the heterogenous population of neurons comprising this ganglion which is vital to sympathetic control of the heart.The methods used in this study are detailed here.
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3159622151