Work

Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

Year: 2021

Type: review

Abstract: Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective "spectroscopic ruler" FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET make it easy to apply and couple with other technologies to comprehensively understand single-molecule dynamics in various application scenarios. Despite its widespread application, smFRET is continuously developing and novel studies based on the advanced platforms have been done. Here, we summarize some representative examples of smFRET research of recent years to exhibit the versatility and note typical strategies to further improve the performance of smFRET measurement on different biomolecules.

Source: Micromachines

DOI: 10.3390/mi12050492

Authors: Yi Qiao, Yuhan Luo, Naiyun Long, Yi Xing, Jing Tu

Institutions: State Key Laboratory of Digital Medical Engineering, Southeast University, Peking University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,944

Funders: National Natural Science Foundation of China, Government of Jiangsu Province, Six Talent Peaks Project in Jiangsu Province, National Key Research and Development Program of China