Work

Sample processing and single cell RNA-sequencing of peripheral blood immune cells from COVID-19 patients

Year: 2021

Type: article

Abstract: Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows in-depth assessment of transcriptional changes in immune cells of patients with COVID-19. However, collecting, processing, and analyzing samples from patients with COVID-19 pose many challenges because blood samples may contain infectious virus, identification of immune cell subtypes can be difficult, and biological interpretation of analytical results is complex. To address these issues, we describe a protocol for sample processing, sorting, methanol fixation, and scRNA-seq analysis of PBMCs from frozen buffy coat samples from patients with COVID-19. For complete details on the use and execution of this protocol, please refer to (Yao et al., 2021).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2021.100582

Authors: Changfu Yao, Stephanie A. Bora, Peter Chen, Helen S. Goodridge, Sina A. Gharib

Institutions: Cedars-Sinai Medical Center, University of Washington

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500