Tumor Cell Invasion into Matrigel: Optimized Protocol for RNA Extraction
Year: 2021
Type: article
Abstract: 3D models are increasingly used to study mechanisms driving tumor progression and mimicking in vitro processes such as invasion and migration. However, there is a need to establish more protocols based on 3D culture systems that allow for downstream molecular biology investigations. Materials & methods: Here we present a method for optimal RNA extraction from highly aggressive primary glioma cells invading into Matrigel. The method has been established by comparing previously reported protocols, available commercial kits and optimizing specific steps for matrix dissociation, RNA separation and purification. Results and conclusion: The protocol allows RNA extraction from cells embedded into Matrigel, with optimal yield, purity and integrity suitable for subsequent sequencing analysis of both high and low molecular weight RNA.
Source: BioTechniques
Authors: Roberta Ferretti, Antonella Baldassarre, Emmanuel de Billy, Angel M Carcaboso, Andrew Moore, Andrea Carai, Angela Mastronuzzi, Andrea Masotti, Maria Vinci
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,415
Funder: CHILDREN with CANCER UK
Award: G2334913331
OpenAlex ID: W3160828928