An optimized and validated method for screening and quantification of commonly encountered stimulant drugs and selected metabolites in dried blood spot (DBS) samples by ultra performance liquid chromatography-quadrupole time of flight-high resolution mass spectroscopy (UPLC QTOF-HRMS) analysis
Year: 2019
Type: dissertation
Abstract: Blood microsampling techniques, like Dried Blood Spots (DBS), have seen recent development \nas an alternative to wet blood samples and offer numerous advantages. Quantification of ng/mLrange concentrations using DBS microvolumes (10-20 µL) is made possible with cutting edge \ninstrumentation. Worldwide establishment of per se limits for drug impaired driving requires \ntimely methods of sampling blood for accurate drug concentration measurements and \ninterpretation. The method validation for the analysis of stimulants and metabolites in DBS \nsamples by Ultra Performance Liquid Chromatography-Quadrupole Time of Flight-High \nResolution Mass Spectroscopy (UPLC-QTOF-HRMS) is presented. Limits of detection and \nquantitation to 10 ng/mL was achieved. Method validation criteria was satisfied for 10 of 14 \nanalytes. DBS drug stability over 8 weeks varied by analyte. DBS samples may assist in \novercoming challenges in blood sampling as they are less invasive, easily transported and stored, \nand accurate drug quantitation even at low concentrations in DBS samples is possible.
Author: Kirk Unger
Language: English
Subfield: Toxicology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3160916771