Work

Insights into the Organization of the Poxvirus Multicomponent Entry-Fusion Complex from Proximity Analyses in Living Infected Cells

Year: 2021

Type: article

Abstract: Poxviruses are enveloped viruses with a DNA-containing core that enters cells following fusion of viral and host membranes. This essential step is a target for vaccines and therapeutics. The entry-fusion complex (EFC) of poxviruses is unusually complex and comprised of 11 conserved viral proteins. Determination of the structure of the EFC is a prerequisite for understanding the fusion mechanism. Here, we used a tripartite split green fluorescent protein assay to determine the proximity of individual EFC proteins in living cells. A network connecting components of the EFC was derived.

Source: Journal of Virology

DOI: 10.1128/jvi.00852-21

Authors: Alexander M. Schin, Ulrike S. Diesterbeck, Bernard Moss

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Language: English

Topic: Poxvirus research and outbreaks

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Division of Intramural Research, National Institute of Allergy and Infectious Diseases