Semi-Automated Extraction of Viral RNA using the MagMax Viral Pathogen (MVPII) 96 Kit for SARS-CoV-2 Detection v1
Year: 2021
Type: preprint
Abstract: The purpose of this protocol is to describe a semi-automated process for viral RNA isolation from anterior nares (AN) swabs in molecular transport medium (MTM), Mawi medium, or 5% sodium dodecyl sulfate (SDS), using the MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit. This procedure applies specifically to samples received for SARS-CoV-2 testing. The MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit uses magnetic bead technology to rapidly isolate, wash, purify, and elute viral RNA from viral samples in transport media. This process is developed for semi-automated systems and performs RNA extraction in a 96-well plate format. The RNA extracted will be used for SARS-CoV-2 detection via a TaqPath COVID-19 Multiplex Real-Time RT-PCR assay (see related protocol). Viral samples are obtained from anterior nares (AN) swabs and placed into MTM, Mawi, or 5% SDS solutions. Viral RNA from these samples is extracted using an Eppendorf epMotion 5075 automated liquid handling workstation and a KingFisher Flex benchtop automated sample preparation machine.
Institution: University of California San Diego
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3165532318