Work

Bone marrow stromal cells (BMSCs CD45 ‐ /CD44 + /CD73 + /CD90 + ) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation

Year: 2021

Type: article

Abstract: Available therapies aimed at treating age‐related osteoporosis are still insufficient. Therefore, designing reliable in vitro model for the analysis of molecular mechanisms underlying senile osteoporosis is highly required. We have isolated and characterized progenitor cells isolated from bone marrow (BMSCs) of osteoporotic mice strain SAM/P6 (BMSC SAM/P6 ). The cytophysiology of BMSC SAM/P6 was for the first time compared with BMSCs isolated from healthy BALB/c mice (BMSC BALB/c ). Characterization of the cells included evaluation of their multipotency, morphology and determination of specific phenotype. Viability of BMSCs cultures was determined in reference to apoptosis profile, metabolic activity, oxidative stress, mitochondrial membrane potential and caspase activation. Additionally, expression of relevant biomarkers was determined with RT‐qPCR. Obtained results indicated that BMSC SAM/P6 and BMSC BALB/c show the typical phenotype of mesenchymal stromal cells (CD44+, CD73+, CD90+) and do not express CD45. Further, BMSC SAM/P6 were characterized by deteriorated multipotency, decreased metabolic activity and increased apoptosis occurrence, accompanied by elevated oxidative stress and mitochondria depolarisation. The transcriptome analyses showed that BMSC SAM/P6 are distinguished by lowered expression of molecules crucial for proper osteogenesis, including Coll‐1 , Opg and Opn . However, the expression of Trap , DANCR1 and miR‐124‐3p was significantly up‐regulated. Obtained results show that BMSC SAM/P6 present features of progenitor cells with disturbed metabolism and could serve as appropriate model for in vitro investigation of age‐dependent osteoporosis.

Source: Journal of Cellular and Molecular Medicine

DOI: 10.1111/jcmm.16667

Authors: Mateusz Sikora, Agnieszka Śmieszek, Krzysztof Marycz

Institution: Wrocław University of Environmental and Life Sciences

Language: English

Topic: Mesenchymal stem cell research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,388