Work

Analysis of Genomic Variation in Different Brassica Napus Synthetic Allopolyploids

Year: 2021

Type: preprint

Abstract: Allopolyploidy is an evolutionary and mechanisticaly intriguing process involving the reconciliation of two or more sets of diverged genomes and regulatory interactions, resulting in new phenotypes. In this study, we explored the genomic variation of eight F2 synthetic B. napus using whole-genome sequencing. We found that there was a genetic variation in the F2 generation. Part of the variation was consistent in the F2 generation, and a small number of mutations only appeared in a single plant of the F2 generation. The analysis of copy number variation (CNV) found that most of the AA genome was lost, and most of the CC genome was obtained. In addition, there was inter-chromosomal translocation (CTX) in the F2 generation and the number of each plant was different. The above results indicate that the F2 generation showed genetic variation and there was a difference between eight plants, which may lay a molecular basis for the unique field performance of the offspring. It provides a new perspective of genomic variation and trait separation in the early stages of allopolyploid polyploid formation.

Source: Research Square

DOI: 10.21203/rs.3.rs-516265/v1