Work

Genetic Incorporation of ϵ‐ N ‐Benzoyllysine by Engineering Methanomethylophilus alvus Pyrrolysyl‐tRNA Synthetase

Year: 2021

Type: article

Abstract: Post‐translational modifications regulate protein structure and function. Lysine benzoylation is a newly discovered histone modification with unique physiological relevance. To construct proteins with this modification site‐specifically, we generated orthogonal tRNA Pyl ‐MaBzKRS pairs by engineering Methanomethylophilus alvus pyrrolysyl‐tRNA synthetase, allowing the genetic incorporation of ϵ‐ N ‐benzoyllysine (BzK) into proteins with high efficiency in E. coli and mammalian cells. Two types of MaBzKRS were identified to incorporate BzK using mutations located at different positions of the amino acid binding pocket. These MaBzKRS are small in size and highly expressed, which will afford broad utilities in studying the biological effects of lysine benzoylation.

Source: ChemBioChem

DOI: 10.1002/cbic.202100218

Authors: Li Cao, Jun Liu, Farid Ghelichkhani, Sharon Rozovsky, Lei Wang

Institutions: University of California, San Francisco, University of Delaware

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Selenoproteins in the ER-associated protein degradation pathway, Adding new covalent bonds to proteins in live cells