Year: 2021
Type: preprint
Abstract: A tightly coordinated developmental program controls precise genetic and metabolic reprogramming that dictates efficient transition of the seeds from dormancy to metabolically active seedlings. Beneficial microbes are known to stimulate the germination of the seeds or adaptation of the seedlings; however, investigations of exact mechanisms mediating these interactions and the resulting physiological responses of the plants are only beginning. Bacillus subtilis is commonly detected in the plant holobiont and belongs to the group of microbes that provide multifaceted contribution to the health of the plants. The present study demonstrated that B. subtilis triggered genetic and physiological responses in the seeds that determined subsequent metabolic and developmental status of adult plants. Chemically diverse extracellular matrix of Bacillus was demonstrated to structurally cooperate in bacterial colonization of the seed storage tissues. Additionally, an amyloid protein and fengycin, which are two components of the extracellular matrix, targeted the oil bodies of the seed endosperm, provoking changes in lipid metabolism or accumulation of glutathione-related molecules that stimulated two different plant growth programs: the development of seed radicles or overgrowth and immunization of adult plants. We propose this mutualistic interaction is conserved in Bacilli and plant seeds containing storage oil bodies.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
Funders: Gordon and Betty Moore Foundation, Ministerio de Ciencia, Innovación y Universidades, Deutsche Forschungsgemeinschaft, Ministerio de Economía y Competitividad, Ministerio de Ciencia e Innovación, National Institutes of Health, Smithsonian Conservation Biology Institute, National Institute of General Medical Sciences, H2020 European Research Council
Awards: G100543663, G1337905801, G1453342619, Discovering novel small molecule antibiotics from complex microbial communities by integrating computational mass spectrometry and metagenome mining, G3197397437, G3690828678, Mechanistic and functional studies of Bacillus biofilms assembly on plants, and their impact in sustainable agriculture and food safety, G4583656673, Reuse of Public Metabolomics Data, Center for Computational Mass Spectrometry, G5555131222, G5825349464, G6240419514, G6878646385, G8900230752, A Platform for Rapidly Identifying Molecules in Symbiotic Interactions, G9000254335, Unified Computation Tools for Natural Products research
OpenAlex ID: W3166922781