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Correction of hematocrit in the analysis of lead (Pb) in dried blood spots using potassium measurements

Year: 2020
Type: erratum
Abstract: Dried blood spots (DBS) is an advantageous sampling tool that has been widely applied in Pb exposure assessment. The benefits of this technique are numerous, including low invasiveness, feasibility, and low costs. Yet, this approach has been challenged by limitations, which prevent this tool to be stablished in Pb biomonitoring programs, such as the “hematocrit (Hct) effect”. The Hct effect refers to how the differences in Hct in the DBS result in an uneven blood spreading through the filter paper. One of the approaches to cope with this effect that has demonstrated reliability, is the use of potassium as a marker of Hct. The goal of this study is to explore the potential of potassium measurements as a correction factor for the Hct effect in the analysis of Pb in DBS, to improve the agreement between whole blood and DBS. We validated a method to assess potassium in DBS using atomic absorption. We built a calibration curve of whole blood samples with a range of Hct levels (0.25-0.60) and a fixed concentration of Pb and we created DBS using an aliquot of these samples. We measured Pb levels and the Hct content (using potassium in the case of DBS) in the whole blood and DBS samples. We obtained a correction factor from the calibration curves of whole blood and DBS. Finally, we evaluated the agreement between whole blood and DBS before and after the correction of Pb concentrations in DBS. The limits of detection and quantification of the method to determine potassium were 1.76 and 5.80 ppm, respectively. The precision and accuracy were 0.02-0.33% and 92.2-124.7%, respectively. The agreement analysis between whole blood and DBS samples before correction showed a bias value of 0.6 µg/dL (LoA: -3.0- 4.2), while the bias after correction was 0.2 µg/dL (LoA: -3.8- 3.4).
DOI: 10.1289/isee.2020.virtual.p-0488
Institution: McGill University
Language: English
Field: Chemistry
Open Access status: diamond
OpenAlex ID: W3169507467
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