Work

In vivo measurement of the lineal density of red blood cells in human retinal capillaries using high-speed adaptive optics ophthalmoscopy

Year: 2021

Type: article

Abstract: We present an automated method for measuring the lineal density of red blood cells (RBCs) in human retinal capillaries using adaptive optics near-confocal ophthalmoscopy (AONCO). The spatiotemporal traces of RBCs flowing in retinal capillaries were extracted from AONCO images, enhanced using the Gabor filter, the vesselness filter, and the Hough transform. A total of 43 capillary segments (each 50 µm long) were analyzed in 12 eyes of 12 subjects, and the measurement error of the automated method was 0.09 cell over 50 µm compared with results obtained by manual counting. Our method provides a tool for assessing RBC spatial distribution in retinal capillaries.

Source: Optics Letters

DOI: 10.1364/ol.428538

Authors: Boyu Gu, David Sarraf, Michael Ip, Srinivas R. Sadda, Yuhua Zhang

Institutions: University of California, Los Angeles, Doheny Eye Institute

Language: English

Topic: Retinal Imaging and Analysis

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: W. M. Keck Foundation, Research to Prevent Blindness, National Institutes of Health, National Eye Institute