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Utilizing the Rheonix NGS OnePrep™ Solution to automate the Takara Bio ThruPLEX® Tag-Seq HV library preparation kit

Year: 2020

Type: article

Abstract: The fully automated Rheonix NGS OnePrep solution streamlined NGS library preparation for the new Takara Bio ThruPLEX Tag-Seq HV kit for Pan-Cancer targeted sequencing. Molecularly tagged, sample-indexed, sequence-ready libraries were produced with the Encompass Optimum workstation and microfluidic Rheonix CARDA cartridge. Data demonstrated that automated and benchtop prepared libraries were equivalent and allowed the detection of low (1%) allele frequency variants. The workflow significantly reduces hands-on time and total time-to-results while increasing sample throughput.

Source: PubMed Central

Authors: H. C. Higgins, Hui Zhu, Beum Jun Kim, Rubina Yasmin, L.D. Sherlin, Julie Laliberté, Patrick R. Martin, Andrew Farmer, Richard A. Montagna

Language: English

Topic: Cancer Genomics and Diagnostics

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W3170916560

FWCI:

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