Work

Total lactate dehydrogenase calorimetric enzyme activity measurement v1

Year: 2021

Type: preprint

Abstract: Lactate Dehydrogenase is a key cytoplasmic enzyme that confers the observed Warburg effect in tumors. Its a tetrameric protein composed of combinations of one or two isomers. This protein catalyzes the reversible reaction This reaction is monitored spectrophotometrically by observing the reduction in absorbance of NADH (which is maximal at 420nm) as pyruvate is converted to lactate. This protocol, details the steps involved in such a calorimetric assay of cells and tissues.

DOI: 10.17504/protocols.io.bvhmn346

Author: Renuka Sriram

Language: English

Topic: Cancer, Hypoxia, and Metabolism

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W3170934001

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