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Methods for in vitro CRISPR/CasRx-Mediated RNA Editing

Year: 2021
Type: article
Abstract: Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the in vitro methods of utilizing this powerful RNA editing platform and determine the RNA editing efficiencies for CasRx with different forms of guide RNAs (also known as gRNA or sgRNA).
DOI: 10.3389/fcell.2021.667879
Authors: Yu-Fan Chuang, Peng-Yuan Wang, Satheesh Kumar, Suraj Lama, Fan-Li Lin, Guei-Sheung Liu
Institutions: University of Tasmania, Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Menzies Institute for Medical Research, The University of Melbourne, Aier Eye Hospital Group (China)
Language: English
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W3170941595
FWCI: 1.051
Cites: 29
Cited by: 22
Related to: 10