Work

Electrochemical Detection of Kinase Activity by Measuring the Surface Charge Change of Peptide Substrate on Gold Electrode

Year: 2021

Type: article

Abstract: Kinase can be used in clinical analysis as the biomarker for disease diagnosis. Herein, we proposed a simple and label-free electrochemical method for kinase detection. The method was based on the charge change of peptide substrate immobilized on the gold electrode. The change was measured by electrochemical impedance spectroscopy and the value was proportional to the concentration of kinase. The method shows satisfactory results for the analysis of kinase inhibitors, thus exhibiting great potential for diagnosis of kinase-related diseases and discovery of novel inhibitor drugs.

Source: International Journal of Electrochemical Science

DOI: 10.20964/2021.07.70

Authors: Lingbin Ou, Yujia Chen, Ning Xia

Institutions: Yongzhou Vocational Technical College, Anyang Normal University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,670

OpenAlex ID: W3171998129

FWCI: 0.067

Cites: 22

Cited by: 1

Related to: 10