Work

An Ultrafast One-Step qRT-PCR Assay for Detection of SARS-CoV-2

Year: 2021

Type: preprint

Abstract: We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 mins on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.

Source: Research Square

DOI: 10.21203/rs.3.rs-582897/v1

Authors: Jadranka Milosevic, Mengrou Lu, Wallace Greene, Hong-Zhang He, Si-Yang Zheng

Institutions: Carnegie Mellon University, Pennsylvania State University, Penn State Milton S. Hershey Medical Center

Language: English

Topic: SARS-CoV-2 detection and testing

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W3172406353

Cites: 11

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Related to: 10