PGFinder, a novel analysis pipeline for the consistent, reproducible, and high-resolution structural analysis of bacterial peptidoglycans
Year: 2021
Type: article
Abstract: Many software solutions are available for proteomics and glycomics studies, but none are ideal for the structural analysis of peptidoglycan (PG), the essential and major component of bacterial cell envelopes. It icomprises glycan chains and peptide stems, both containing unusual amino acids and sugars. This has forced the field to rely on manual analysis approaches, which are time-consuming, labour-intensive, and prone to error. The lack of automated tools has hampered the ability to perform high-throughput analyses and prevented the adoption of a standard methodology. Here, we describe a novel tool called PGFinder for the analysis of PG structure and demonstrate that it represents a powerful tool to quantify PG fragments and discover novel structural features. Our analysis workflow, which relies on open-access tools, is a breakthrough towards a consistent and reproducible analysis of bacterial PGs. It represents a significant advance towards peptidoglycomics as a full-fledged discipline.
Source: eLife
DOI: 10.7554/elife.70597
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,500
Awards: G1979782309, G2193730806, G282427092, Mechanism of secretion of large clostridial toxins , Deciphering peptidoglycan structural patterns and host responses in intracellular bacterial infections, G3256177976, G335622025, G4656594441, G5420063140, G6942171995, G7240651115, G7376787254, G7437391126, How does microbiota-born peptidoglycan affect host homeostasis?, Analysis of the L,D transpeptidation pathway in Clostridium difficile: contribution to peptidoglycan synthesis and antibiotic resistance, White Rose Doctoral Training Partnership in Mechanistic Biology and its Strategic Application, G8670608220
OpenAlex ID: W3202917468