Centriole and Golgi microtubule nucleation are dispensable for the migration of human neutrophil-like cells
Year: 2021
Type: article
Abstract: Neutrophils migrate in response to chemoattractants to mediate host defense. Chemoattractants drive rapid intracellular cytoskeletal rearrangements including the radiation of microtubules from the microtubule-organizing center (MTOC) toward the rear of polarized neutrophils. Microtubules regulate neutrophil polarity and motility, but little is known about the specific role of MTOCs. To characterize the role of MTOCs on neutrophil motility, we depleted centrioles in a well-established neutrophil-like cell line. Surprisingly, both chemical and genetic centriole depletion increased neutrophil speed and chemotactic motility, suggesting an inhibitory role for centrioles during directed migration. We also found that depletion of both centrioles and GM130-mediated Golgi microtubule nucleation did not impair neutrophil directed migration. Taken together, our findings demonstrate an inhibitory role for centrioles and a resilient MTOC system in motile human neutrophil-like cells.
Source: Molecular Biology of the Cell
Institution: University of Wisconsin–Madison
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Awards: Impact of chromosomal instability on sensitivity to microtubule-targeting drugs in breast cancer, Identifying causes and consequences of centrosome amplification in cancer, Training Program In Molecular AND Cellular Pharmacology, Integrated Training For Physician-Scientists, Microscale models of inflammation and its resolution, UW Comprehensive Cancer Center Support, Research Training in Hematology
OpenAlex ID: W3173949329