Year: 2019
Type: preprint
Abstract: Generation of Tn5 transposome, protein purification and loading for the sci- family of protocols. Protein purification of pTXB1.Tn5 transformed into T7 Express LysY (NEB C3031) cells; reference is PTXB1-Tn5 Addgene #60240 from Picelli et al. Genome Res. 2014.'' This protocol is built largely off of work described in Picelli, Simone, et al. "Tn5 transposase and tagmentation procedures for massively scaled sequencing projects." Genome research 24.12 (2014): 2033-2040.
Authors: Ryan Mulqueen, Andy Fields, Andrew Adey
Institution: Oregon Health & Science University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3174393992