Work

Separation of presynaptic Ca v 2 and Ca v 1 channel function in synaptic vesicle exo- and endocytosis by the membrane anchored Ca 2+ pump PMCA

Year: 2021

Type: article

Abstract: Significance Synaptic vesicle (SV) release from presynaptic terminals requires nanometer precise control of action potential (AP)–triggered calcium influx through voltage-gated calcium channels (VGCCs). SV recycling also depends on calcium signals, though in different spatiotemporal domains. Mechanisms for separate control of SV release and recycling by AP-triggered calcium influx remain elusive. Here, we demonstrate largely independent regulation of release and recycling by two different populations of VGCCs (Ca v 2, Ca v 1), identify Ca v 1 as one of potentially multiple calcium entry routes for endocytosis regulation, and show functional separation of simultaneous calcium signals in the nanometer space of a presynaptic terminal by the plasma membrane calcium ATPase (PMCA). The Ca v 2/Ca v 1/PMCA functional triad may provide conserved means for independent control of different vital presynaptic functions.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.2106621118