Work

Receptor Switching in Newly Evolved Adeno-associated Viruses

Year: 2021

Type: article

Abstract: Understanding how viruses interact with host cells through cell surface receptors is central to discovery and development of antiviral therapeutics, vaccines, and gene transfer vectors. Here, we demonstrate that distinct epitopes on the surface of adeno-associated viruses can be evolved by infectious cycling to recognize different cell surface carbohydrates and glycoprotein receptors and solve the three-dimensional structure of one such newly evolved AAV capsid, which provides a roadmap for designing viruses with improved attributes for gene therapy applications.

Source: Journal of Virology

DOI: 10.1128/jvi.00587-21

Authors: L. Patrick Havlik, Anshuman Das, Mario Mietzsch, Daniel K. Oh, Jonathan Ark, Robert McKenna, Mavis Agbandje-McKenna, Aravind Asokan

Institutions: University of North Carolina at Chapel Hill, Duke University, Duke Medical Center, University of Florida

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke, National Institute of Arthritis and Musculoskeletal and Skin Diseases

Awards: CircRNAs and CNS Gene Transfer, G3330158216, West/Midwest Consortium for High-Resolution Cryo Electron Microscopy, The Southeastern Consortium for Microscopy of MacroMolecular Machines, RE-ENGINEERING AAV GENOME PACKAGING, Evolving High Potency AAV Vectors for Neuromuscular Genome Editing, AAV capsids and their cellular interactions, Determinants of AAV Tropism

OpenAlex ID: W3178816563

FWCI: 1.975

Cites: 64

Cited by: 24

Related to: 10