Year: 2021
Type: review
Abstract: Fluorescent probes are powerful tools for visualizing cellular and subcellular structures, their dynamics and cellular molecules in living cells and enable us to monitor cellular processes in a spatiotemporal manner within complex and crowded systems. In addition to popular fluorescent proteins, a wide variety of small-molecule dyes have been synthesized through close association with the interdisciplinary field of chemistry and biology, ranging from those suitable for labeling cellular compartments such as organelles to those for labeling intracellular biochemical and biophysical processes and signaling. In recent years, self-labeling technologies including the SNAP-tag system have allowed us to attach these dyes to cellular domains or specific proteins and are beginning to be employed in plant studies. In this mini review, we will discuss the current range of synthetic fluorescent probes that have been exploited for live-cell imaging and the recent advances in the application that enable genetical tagging of synthetic probes in plant research.
Source: Plant and Cell Physiology
DOI: 10.1093/pcp/pcab104
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,150
Awards: Functional relationship between microtubule nucleation and severing in plant cell division, Dissecting role of sucrose and GA recognition in SWEET transporters, G2642650165, G2958780423, G4378662817, EXC 2048: CEPLAS Exzellenzcluster für Pflanzenwissenschaften, G7735813670, Development of genetically-encoded voltage indicator for plants
OpenAlex ID: W3181553285