The Blocking of TNF-alpha by RNA Interference and Its Influence on Synovial Fibroblast and Chondrocytes
Year: 2007
Type: article
Abstract: Objective: Small interfering RNA (siRNA) triggers RNA interference in mammalian somatic cells.TNF-α is a proinflammatory cytokine implicated in the pathogenesis of inflammatory arthritis including rheumatoid arthritis (RA).This study was to use TNF receptor 1 (TNFRI)specific siRNA to inhibit the TNF-α mediated signaling in RA fibroblast like synoviocytes (FLS) and chondrocytes.Methods: TNFRI specific siRNA was produced by targeting 3 nucleotide sequences at 474∼ 494, 562∼582 and 668∼688.Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were performed to optimize the silencing effects of TNFRI siRNA in cultured FLS and chondrocytes.The inhibition of TNF-α mediated signaling was determined by ELISA assay of metalloproteinase 1 secretion induced by TNF-α. Results:The TNFRI siRNA inhibited the expression of TNFRI mRNA and protein in both RA FLS and chondrocytes.MMP-1 secretion induced by TNF-α was significantly downregulated by TNFRI siRNA.Conclusion: TNFRI siRNA can inhibit the expression and signaling downstream of TNFRI
Source: Journal of Rheumatic Diseases
Author: Hyun Ah Kim
Institution: Hallym University Sacred Heart Hospital
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2085604902