Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid
Year: 2021
Type: article
Abstract: Glycerol phosphate (GroP)-based teichoic acids (TAs) are antigenic cell-wall components found in both enterococcus and staphylococcus species. Their immunogenicity has been explored using both native and synthetic structures, but no details have yet been reported on the structural basis of their interaction with antibodies. This work represents the first case study in which a monoclonal antibody, generated against a synthetic TA, was developed and employed for molecular-level binding analysis using TA microarrays, ELISA, SPR-analyses, and STD-NMR spectroscopy. Our findings show that the number and the chirality of the GroP residues are crucial for interaction and that the sugar appendage contributes to the presentation of the backbone to the binding site of the antibody.
Source: ACS Chemical Biology
Authors: Francesca Berni, Ermioni Kalfopoulou, Ana M. Gimeno Cardells, Filippo Carboni, Daan van der Es, Felipe Romero-Saavedra, Diana Laverde, Karmela Miklic, Suzana Malic, Tihana L. Rovis, Stipan Jonjic, Sara Ali, Herman S. Overkleeft, Cornelis H. Hokke, Angela van Diepen, Roberto Adamo, Jesús Jiménez-Barbero, Gijsbert A. van der Marel, Johannes Huebner, Jeroen D. C. Codée
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $5,000
Award: G595234094
OpenAlex ID: W3182176838