Work

Role of the Staphylococcus aureus Extracellular Loop of GraS in Resistance to Distinct Human Defense Peptides in PMN and Invasive Cardiovascular infections

Year: 2021

Type: article

Abstract: was comparable at pH 5.5 between the P38G and D35/37/41K point mutants, indicating a mechanistic divergence between GraS activation by acidic pH versus cationic peptides. Survival assays in human blood and purified polymorphonuclear leukocytes (PMNs) revealed lower survival of the D35/37/41K mutant versus the F38G mutant, with both being lower than that of the parent. Virulence studies in the rabbit endocarditis model mirrored whole blood and PMN killing assay data described above. Collectively, these data confirmed the importance of specific residues within the EL of GraS in conferring essential bacterial responses for MRSA survival in infections.

Source: Infection and Immunity

DOI: 10.1128/iai.00347-21

Authors: Ambrose L. Cheung, Junho Cho, Arnold S. Bayer, Michael R. Yeaman, Yan Q. Xiong, Niles P. Donegan, Irina V. Mikheyeva, Gi Yong Lee, Soo-Jin Yang

Institutions: Dartmouth College, University of California, Los Angeles, The Lundquist Institute, Harbor–UCLA Medical Center, Seoul National University, Research Institute for Veterinary Science

Language: English

Topic: Antimicrobial Resistance in Staphylococcus

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: HCF Research Foundation, National Research Foundation of Korea, National Institutes of Health, Audrey and Theodor Geisel School of Medicine at Dartmouth, National Institute of Allergy and Infectious Diseases

Awards: Mitigating Resistance & Virulence in MRSA, The role of GraRS in resistance to cationic antimicrobial peptides in S. aureus, The role of purine biosynthesis and stringent response in persistent MRSA endovascular infections, Mitigating Resistance & Virulence in MRSA, G6435858978, Mechanisms of Staphylococcal Co-Resistance to Daptomycin and Host Defense Peptide, Systems Immunolobiology of Antibiotic-Persistent MRSA Infection, G9000111704

OpenAlex ID: W3182312717

FWCI: 0.633

Cites: 41

Cited by: 10

Related to: 10