Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and testis
Year: 2021
Type: article
Abstract: Endothelial cells (ECs) exhibit phenotypic and functional tissue specificities, critical for studies in the vascular field and beyond. Thus, tissue-specific methods for isolation of highly purified ECs are necessary. Kidney, spleen, and testis ECs are relevant players in health and diseases such as chronic kidney disease, acute kidney injury, myelofibrosis, and cancer. Here, we provide tailored protocols for rapid and reproducible EC purification established for scRNA sequencing from these adult murine tissues using the combination of magnetic- and fluorescence-activated cell sorting. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020) 5. Kalucka, J. ∙ De Rooij, L.P.M.H. ∙ Goveia, J. ... Single-cell transcriptome atlas of murine endothelial cells Cell. 2020; 180 :764-779.e20 Google Scholar and Dumas et al. (2020) 3. Dumas, S.J. ∙ Meta, E. ∙ Borri, M. ... Single-cell RNA sequencing reveals renal endothelium heterogeneity and metabolic adaptation to water deprivation J. Am. Soc. Nephrol. 2020; 31 :118 Google Scholar .
Source: STAR Protocols
Authors: Sébastien J. Dumas, Elda Meta, Nadine V. Conchinha, Liliana Sokol, Rongyuan Chen, Mila Borri, Laure-Anne Teuwen, Koen Veys, Melissa García-Caballero, Vincent Geldhof, Lucas Treps, Pauline de Zeeuw, Kim D. Falkenberg, Charlotte Dubois, Magdalena Parys, Laura P.M.H. de Rooij, Katerina Rohlenova, Jermaine Goveia, Luc Schoonjans, Mieke Dewerchin, Guy Eelen, Xuri Li, Joanna Kalucka, Peter Carmeliet
Institutions: VIB-KU Leuven Center for Cancer Biology, KU Leuven, Sun Yat-sen University, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, University of Antwerp, Université de Lille, GZA Ziekenhuizen Campus Sint-Augustinus, GZA Ziekenhuizen, Inserm, Memorial Sloan Kettering Cancer Center, Aarhus University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Funders: Aarhus Universitet, European Commission, Aarhus Universitets Forskningsfond, Carlsbergfondet, Fonds Wetenschappelijk Onderzoek, Lundbeckfonden, Novo Nordisk, Vlaams Instituut voor Biotechnologie, Stichting Tegen Kanker, A.P. Møller og Hustru Chastine Mc-Kinney Møllers Fond til almene Formaal, Riisfort Fonden, Universiteit Antwerpen, Novo Nordisk Fonden, Kom op tegen Kanker, Vlaamse regering, Fondation contre le Cancer, European Research Council, H2020 European Research Council, H2020 Marie Skłodowska-Curie Actions, Steno Diabetes Center Aarhus
OpenAlex ID: W3183407644