Recapitulating Nutritionally Regulated Human Muscle Protein Metabolism In vitro using Humanized Cell Media
Year: 2021
Type: dissertation
Abstract: Most in vitro models used to study the effects of nutrients on skeletal muscle protein metabolism use supraphysiological concentrations of nutrients and amino acids, making translation of their findings to humans difficult. Therefore, there is a need to develop an in vitro model that uses physiological nutrient concentrations. This study used a humanized fasted and fed media, modeled after human plasma fasted and fed amino acid concentrations, to study in vitro protein metabolism and anabolic signalling following nutrient withdrawal and refeeding compared to typical supraphysiological withdrawal and refeeding. In all outcomes measured, there was a similar knockdown of protein synthesis and anabolic signalling in both models. Further, refeeding generally resulted in similar phosphorylation responses in the proteins measured despite protein synthesis differing between the two models. These findings suggest that a humanized media may be a useful tool to study skeletal muscle physiology in vitro.
Source: TSpace (University of Toronto)
Author: Cassidy T. Tinline‐Goodfellow
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3183967566