Long Non-Coding RNA LINC-01572:28 Inhibits Granulosa Cell Growth Via a Decrease in p27 (Kip1) Degradation in Patients with Polycystic Ovary Syndrome
Year: 2018
Type: article
Abstract: Background Disordered folliculogenesis is a key feature of polycystic ovary syndrome (PCOS), but the underlying molecular mechanism remains unclear. Methods Long non-coding RNA (lncRNA) expression in luteinized granulosa cells (hLGCs) derived from women with and without PCOS were analyzed using microarray and qRT-PCR. Immortalized human granulosa cell lines were cultured for proliferation assays after transfection with the LINC-01572:28 over-expression vector in the presence or absence of p27 siRNA. Protein expression analysis, rescue assays, and RNA immunoprecipitation (RIP) were used to confirm the LINC-01572:28 substrate. Findings LINC-01572:28 and p27 protein were elevated whereas proliferating cell nuclear antigen protein was decreased in the hLGCs of women with PCOS. LINC-01572:28 expression was positively correlated with basal testosterone levels. Over-expression of LINC-01572:28 inhibited cell proliferation and impeded G1/S transition, which were partially reversed by siRNA-mediated p27 knockdown. Interpretation Our findings, therefore, suggest that LINC-01572:28 suppresses cell proliferation and cell cycle progression by reducing the degradation of p27 protein via SKP2 binding.
Source: EBioMedicine
Authors: Jun Zhao, Jieying Xu, Wangshen Wang, Han Zhao, Hongbin Liu, Xiaojing Liu, Jiansheng Liu, Yun Sun, Andrea Dunaif, Yanzhi Du, Zi-Jiang Chen
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $5,780
Funders: National Natural Science Foundation of China, Shanghai Municipal Education Commission, Chinese University of Hong Kong, Shanghai Key Laboratory of Embryo and Reproduction Engineering, National Institutes of Health, National Key Research and Development Program of China, Research and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1435027050, G2077825186, Genes, Androgens and Intrauterine Environment in PCOS, G2476426523, G3421647580, G3720696785, G3763734153, G4106989883, Functional Electrical Stimulation and Volitional Effort Training, G5521443489, G6585006997, G7023521554, Multiethnic Fine-Mapping of Polycystic Ovary Syndrome Susceptibility Loci, G7446663464
OpenAlex ID: W2895433018