Multiplex Mass Spectrometry Analysis of Amyloid Proteins in Human Plasma for Alzheimer’s Disease Diagnosis
Year: 2021
Type: article
Study design: Observational Study
Abstract: Direct analysis of amyloid proteins in human plasma will promote rapid screening of brain amyloidosis, the earliest pathological signature of Alzheimer's disease. We developed a microflow liquid chromatography-targeted mass spectrometry assay for quantitation of four intact β-amyloid proteins starting from 1 mL of human plasma samples. This method showed 90% accuracy for predicting brain amyloid using plasma Aβ42/Aβ40 values from 36 cognitively normal individuals in a prospective clinical study (raw data deposited in MassIVE, Data set ID MSV000087451). Our method may contribute to early diagnosis of Alzheimer's disease.
Source: Journal of Proteome Research
Authors: Weimin Ni, William Jagust, Daojing Wang
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: AD-MS Chips for Biofluids-Based Diagnosis of Alzheimer's Disease, Senescence-Chips for Radiation Biodosimetry, G2749707749, Picoelectrospray Ionization Mass Spectrometry for Top-down Proteomics, G2795071984, G3089326124, Picoelectrospray Ionization Mass Spectrometry for Top-down Proteomics, G4597545058, G5094328609, G6778851630, G6815549504, AD-MS Chips for Biofluids-Based Diagnosis of Alzheimer's Disease, G8172376892, G8374358241, Microfluidic Chips for Biomonitoring
OpenAlex ID: W3186421747