Work

Heterogeneity of immune cells in human atherosclerosis revealed by scRNA-Seq

Year: 2021

Type: review

Abstract: Immune cells in atherosclerosis include T, B, natural killer (NK) and NKT cells, macrophages, monocytes, dendritic cells (DCs), neutrophils, and mast cells. Advances in single-cell RNA sequencing (sRNA-Seq) have refined our understanding of immune cell subsets. Four recent studies have used scRNA-Seq of immune cells in human atherosclerotic lesions and peripheral blood mononuclear cells (PBMCs), some including cell surface phenotypes revealed by oligonucleotide-tagged antibodies, which confirmed known and identified new immune cell subsets and identified genes significantly up-regulated in PBMCs from HIV+ subjects with atherosclerosis compared to PBMCs from matched HIV+ subjects without atherosclerosis. The ability of scRNA-Seq to identify cell types is greatly augmented by adding cell surface phenotype using antibody sequencing. In this review, we summarize the latest data obtained by scRNA-Seq on plaques and human PBMCs in human subjects with atherosclerosis.

Source: Cardiovascular Research

DOI: 10.1093/cvr/cvab260

Authors: Jenifer Vallejo, Clément Cochain, Alma Zernecke, Klaus Ley

Institutions: La Jolla Institute for Immunology, Universitätsklinikum Würzburg, University of California San Diego

Language: English

Topic: Atherosclerosis and Cardiovascular Diseases

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Deutsche Forschungsgemeinschaft, National Heart, Lung, and Blood Institute