Year: 2019
Type: dissertation
Abstract: This study investigated the effects of the ketone body -hydroxybutyrate (HB) on glucose and fat metabolism in skeletal muscle cells. To accomplish this, L6 muscle cells were incubated with various HB concentrations (0.5, 1.0, 1.5, 20, and 2.5 mM) for 1, 3, and 5 days. Here, we report that HB significantly increased glucose uptake and its metabolism in L6 muscle cells. Time course analysis revealed that as early as 1 day of exposure to 1mM HB significantly enhanced basal and insulin-stimulated glucose uptake (1.6-fold), glycogen synthesis (1.3-fold), glucose oxidation (1.5-fold), and lactate production (1.3-fold) in skeletal muscle cells. Even though carnitine palmitoyl transferase 1 (CPT1) content and AMP-activated protein kinase (AMPK) phosphorylation significantly increased, no alteration was found in palmitate oxidation with HB treatment. Surprisingly, protein kinase B (AKT) phosphorylation was significantly reduced in muscle cells exposed to HB. In summary, HB increased AMPK phosphorylation and enhanced glucose uptake without altering fatty acid oxidation in skeletal muscle cells.
Source: YorkSpace (York University)
Author: Ishvinder Hadday
Language: English
Topic: Diet and metabolism studies
Subfield: Physiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W3189223628