LncRNA HCG18 promotes cell multiplication and metastasis by miR-148b/ETV5 regulation in osteosarcoma.
Year: 2021
Type: article
Abstract: OBJECTIVE: To determine the effects of long non-coding RNA (LncRNA) HCG18 on cell multiplication and invasion of osteosarcoma. METHODS: MTT assay and transwell assay were used for cell multiplication and invasion, respectively. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot were used to determine transcriptional and translational expression. Luciferase reporter assay was used to identify the specific target relationships. RESULTS: , which was achieved by down-regulating the overexpression of miR-148b, and down-regulating ETV5, indicating combination of ETV5 and miR-148b in osteosarcoma. Overexpression of ETV5 could reverse the inhibitory effect of knockout of lncRNA HCG18 on cell multiplication and invasion. CONCLUSION: LncRNA HCG18 acted as a sponge of miR-148b and played an oncogenic role in osteosarcoma, providing therapeutic targets for osteosarcoma.
Source: PubMed
Authors: Zhipan Zheng, Kai Lin
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3190194286